Fabio Zanini on ASeq Newsletter

Thank you for the article. Just wanted to comment quickly to help your analysis. If you are on the hunt for sensitivity, you would do ddPCR or smFISH, not qPCR. Both method have single molecule sensitivity given you are able to extract a single molecule of viral nucleic acid from a nasal swab. The efficiency of the extraction itself is highly variable depending on how degraded it is, time since isolation, storage buffer, etc but is independent of the detection method. qPCR is just quick and easy, that's why it's popular. No need to mess with a keyboard. Sensitivity is not really the point there, usually.