How do you make 500ml of 1M Tris? - Answers

To make 500 ml of a 1M Tris (tris(hydroxymethyl)aminomethane) solution, you need to dissolve 61.44 grams of Tris base in distilled water. First, weigh out the required amount of Tris, then add it to a container with approximately 400 ml of distilled water. Stir until fully dissolved, and then adjust the final volume to 500 ml with additional distilled water. Optionally, you can adjust the pH to your desired value, typically around 7.5, using hydrochloric acid or sodium hydroxide.