How do you prepare 10mM b mercaptoethanol? - Answers

To prepare a 10 mM solution of β-mercaptoethanol, first calculate the required volume of β-mercaptoethanol needed based on its molecular weight (approximately 78.13 g/mol). For example, to make 100 mL of a 10 mM solution, you would need 0.78 mL of β-mercaptoethanol. Measure this amount using a micropipette or a graduated cylinder and then dilute it with distilled water to a final volume of 100 mL in a suitable container. Always handle β-mercaptoethanol in a fume hood and use appropriate personal protective equipment, as it is a toxic and malodorous substance.