How does an apoenzyme form? - Answers

An apoenzyme forms when a protein component, known as the apoenzyme, is synthesized in the cell without its necessary cofactors or coenzymes. These cofactors, which can be metal ions or organic molecules, are essential for the enzyme's activity. Once the apoenzyme encounters and binds to its specific cofactor, it undergoes a conformational change, becoming an active holoenzyme capable of catalyzing biochemical reactions. This process is crucial for the proper functioning of many enzymes in metabolic pathways.